Single Nucleus Dispensing

Single-cell RNA-sequencing (scRNA-seq) has been widely adopted for investigating gene expression in individual cells, offering unparalleled resolution into cell type-specific genomic identities. Recently, single nucleus RNA-sequencing (snRNA-seq) has emerged as a valuable alternative to scRNA-seq, allowing researchers to capture mRNA transcripts from individual nuclei. This approach is compatible with frozen cell or tissue samples and additionally reduces sample dissociation biases as well as dissociation-induced transcriptional stress responses. Namocell’s single cell dispensers offer a high throughput, benchtop solution to single cell isolation for plate-based genomics applications, such as scRNA-seq. Learn how a Namocell dispenser was used to isolate single DNA-labeled nuclei to demonstrate compatibility with snRNA-seq workflows.