Simplify Cell Detachment Processes With Serum-Free Media

By Anna-Barbara Hachmann, Andrew M. Campbell, and Stephen F. Gorfien

Vaccine production with adherent cell lines faces multiple challenges which include selection of a suitable vessel, detachment of cells for scale up, optimization of infection, as well as harvest of virus particles. In this study we identified ways to simplify the process and evaluated stirring techniques to measure the viable cell densities for the production of the rabies virus surrogate, vesicular stomatitis virus, in Vero cells.