Simplified Lentivirus And AAV Clarification With TFDF®-Based Intensified Virus Production

Biopharmaceutical manufacturers constantly seek innovative methods to enhance viral vector production for gene therapies. Previous research has highlighted the benefits of Tangential Flow Depth Filtration (TFDF^®) for perfusion cell culture, demonstrating its potential to significantly increase viral vector yields.

Now, a new study delves into the critical downstream process of clarification post-TFDF, revealing further advantages of this intensified approach. This research demonstrates that TFDF not only boosts virus production, but also streamlines downstream processing, leading to simplified and more efficient filtration at commercial scale. By retaining cells and most fine debris, TFDF reduces the burden on subsequent clarification steps, potentially minimizing the required filter surface area. This study meticulously characterizes the clarification process following TFDF, identifying optimal filter sizes and types to ensure complete clarification, high virus yield, efficient throughput, and effective turbidity reduction.

Want to learn how TFDF can revolutionize your viral vector manufacturing process? Access the application note to discover the details of this groundbreaking research.