Seed Culture Expansion Process: Reduce Time And Maximize Utilization

Seed culture expansion is commonly performed in several consecutive batch cultures. Starting from a cryopreserved cell stock, the initial culture expansion is typically performed in shake flasks, while the final steps are performed in bioreactor cultures. Each expansion step commonly takes three to four days and, unless using a culture vessel with a large turn-down ratio, a new culture vessel is needed for each volume increase. Such a procedure is time-consuming and labor intensive, and each manual interaction constitutes a risk of contamination of the culture.

With perfusion culturing, on the other hand, cells can be maintained in exponential growth phase for an extended period of time and reach higher viable cell densities compared with batch and fed-batch culturing