Screening Of Chemically-Defined Media For Suspension Cultures Of HEK293 Cells

There has been a desire to improve both process productivities and consistencies of the adherent HEK293 cells grown in serum-supplemented media. As a result, processes have been adapted for culturing of HEK293 cells in suspension and with serum-free, chemically-defined cell media.

In this application note we demonstrate how researchers can utilize a quick media screen to survey their cell media options before investing in a media platform for their HEK293 suspension processes. Carrying this type of analysis forward whenever a fresh media batch is received or periodically during storage, would establish a historical record to refer to in case any unexpected variations arise in the culture’s health and productivity.