Scale-Up Of CHO Cell Fed-Batch Cultures In HyClone™ ActiPro™ Medium Supplemented With Cell Boost™ 7a And 7b

The most widely used cell lines for biopharmaceutical production of therapeutic proteins originate from CHO cells. These cells are robust in culture and are able to produce a variety of recombinant glycoproteins at high levels in large scales. ActiPro medium and Cell Boost 7a and 7b supplements are designed and optimized for largescale protein production in fed-batch suspension culture using recombinant CHO cells. Chemically defined with no peptides or hydrolysates, ActiPro medium and Cell Boost supplements are animal-derived component-free (ADCF) and manufactured using current good manufacturing practices (cGMP). As ActiPro medium and Cell Boost 7a and 7b supplements do not contain hypoxanthine or thymidine (HT), their formulations supports the dihydrofolate reductase (DHFR) gene amplification and selection system. ActiPro medium and Cell Boost 7a and 7b supplements can be used together in a fed-batch process or the medium and supplements can be used in combination with other products to increase productivity of an existing process.

This application note demonstrates growth and productivity of CHO cells cultured in ActiPro medium and Cell Boost 7a and 7b supplements. Studies were performed with multiple CHO cell clones in 30 mL shake flasks cultures. To demonstrate scalability of the process, cultures were transferred to 2 L and 50 L bioreactor cultures and cell growth and productivity of selected clones were compared between scales.