Application Note

S.U.B. Enhancements For High-Density Perfusion Cultures

Bioreactor

Design improvements, performance characterization, modeling, and cell culture

Improvements in single-use systems have allowed implementation of high-density cultures in emerging bioprocess workflows, while progressive advances in media optimization and improved clone genetic selection have underscored the perceived performance limitations of single-use bioreactors (S.U.B.s). This study presents how strategic enhancements to the sparge and agitation systems of Thermo Scientific™ HyPerforma™ S.U.B.s have revealed the potential for a three- to four-fold improvement in mixing and mass transfer performance compared to legacy S.U.B. designs. This study investigates the following:

  • Bioreactor characterization, Thermo Scientific™ TruBio™ DeltaV™ controller optimization, online process analytics, and scalability analysis of the S.U.B. when targeting perfusion applications in working volumes for pilot scale (50 L) to production scale (500 L).
  • High-density culture results (>260 x 106 cells/mL) while maintaining proper operating parameters. New data reveal how a 50 L S.U.B. equipped with a specialized precision drilled-hole sparger (DHS), single-use foam probe, and oversized impeller is able to improve overall S.U.B. operating efficiency. Results also include specific suggestions on how to maintain a nearly ideal dissolved carbon dioxide environment, reduce generation of foam in the  eadspace, and produce lower overall shear levels, thus yielding excellent cell viability.
VIEW THE APPLICATION NOTE!
Signing up provides unlimited access to:
Signing up provides unlimited access to:
  • Trend and Leadership Articles
  • Case Studies
  • Extensive Product Database
  • Premium Content
HELLO. PLEASE LOG IN. X

Not yet a member of BioProcess Online? Register today.

ACCOUNT SIGN UP X
Please fill in your account details
Login Information
I'm interested in newsletter subscriptions.
ACCOUNT SIGN UP

Subscriptions

Sign up for the newsletter that brings you the industry's latest news, technologies, trends and products.

You might also want to: