Quantification Of Total mRNA Content In LNP Complexes Using The SoloVPE® Variable Pathlength System

By Paul Mania, Bioanalytics Applications Specialist, Repligen, Travis Alvine, Sr. Manager Analytical Method Validation, Aldevron, and Natalie Unsinn, Sr. Analyst Method Validation, Aldevron

Accurate quantification of mRNA within lipid nanoparticle (LNP) complexes is a critical step in developing and manufacturing mRNA-based therapeutics. Explore a streamlined approach for determining total mRNA content without relying on destructive or time-intensive methods, as well as how precise measurement ensures proper dosing, formulation consistency, and regulatory compliance—key factors for successful scale-up and commercialization. Gain insight into practical considerations for integrating rapid, non-destructive analysis into workflows, reducing variability, and accelerating decision-making. For scientists and process engineers working in mRNA vaccine or therapeutic development, understanding these techniques is essential for improving efficiency and safeguarding product integrity.

Access the full application note to learn how advanced optical methods can simplify mRNA quantification and support robust LNP characterization.