Comparison of production efficiency with stainless steel tank fermentor
Advances in single-use bioprocess technology have led to the development of the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.), the first specifically designed fermentor of its kind. This S.U.F. equipment has since been integrated into our cGMP production facility in Lithuania. This study was undertaken to demonstrate the feasibility of technical transfer of procedures for routine production of an anti–human IgG antibody fragment in Saccharomyces cerevisiae, from a stainless steel vessel to the HyPerforma S.U.F. Procedural timing for the culture remained unchanged, and only small adjustments were made to agitation and gas-flow settings. For simplicity, the S.U.F. nutrient medium was sterile-filtered and the stainless steel vessel with medium was steam-sterilized. Results showed that production of the native antibody fragment and titers in the S.U.F. were comparable to those of the stainless steel fermentor.