Probing Formulation Differences In Super Optimal Broth With Catabolite Repression Media

Super Optimal Broth with catabolite repression (SOC) media use maximizes the transformation efficiency of competent cells (e.g., E. coli). The added glucose makes SOC media an optimal choice to ensure the metabolic health of bacteria after transformation. Despite its implementation, there can be variations in the individual component concentrations between different media’s commercial sources. This can be due to varying sources of raw materials or preparation procedures during manufacturing. These changes may affect the culture’s performance after transformation, especially if processes have been established based on another vendor’s media.

Read how testing media before the start of culture ensures that nutrients in a media are at a desirable level.