Overcoming Scale-Up Challenges In Viral Vector TFF: Custom System Design And Flexible Workflow Solutions

Scaling up Tangential Flow Filtration (TFF) for viral vector manufacturing from lab scale to $50+$ L presents significant hurdles, particularly around maximizing product yield, expanding system capacity, and ensuring cGMP compliance and automation integration1.

For high-value viral vectors, minimizing unrecoverable volume is critical for maximizing yield2222. A custom TFF system design can overcome these challenges by using compact fluid paths and strategic sensor placement to reduce hold-up volume to under $500\text{ ml}$3. Furthermore, incorporating a single-use magnetic mixer as a feed tank and single-use containers with load cells for permeate collection enables a scalable design with precise volume control4. Finally, using a configurable software interface that supports open communication protocols ensures seamless integration with existing upstream and downstream operations while maintaining regulatory confidence through 21 CFR Part 11 compliance5555.

Discover how a tailored TFF solution delivers a scalable, high-yield process that integrates smoothly with existing infrastructure and maintains regulatory compliance.