Overcoming Poor Cell Recovery And Low Viability Following Cryopreservation And Revival To Accelerate The Development Of New Protein Therapeutics

Cryopreservation and revival of cells induce stress and often result in low viability and poor cell recovery. Whilst Foetal Bovine Serum (FBS) is well established in its ability to support cells, its application raises regulatory concerns due to the increased risk of contamination of health products, and batch variability.

With FBS out of the picture, how do we support cells during cryopreservation and revival to accelerate workflows and reduce costs? InstiTHAW CHO is an animal component-free cell culture supplement that is specifically designed to improve the recovery of cells, enabling cell line development campaigns to commence with haste following host cell revival. When it comes to master cell banking, InstiTHAW CHO enables top-producing clones to be banked and revived with confidence. The product is simply added to the cryopreservative mix and cell culture medium during revival to support the health of the cells. In this video, a scientist from Advanced Instruments demonstrates how InstiTHAW CHO is used to support cells during cryopreservation and thawing.