Overcoming Cloning Inefficiencies To Accelerate The Development Of New Protein Therapeutics

Single cell cloning for regulatory approval is often considered one of the most challenging steps in cell line development. While Foetal Bovine Serum (FBS) is well established in its ability to support the growth of single cells, its application raises regulatory concerns due to the increased risk of contamination of health products, and batch variability.

With FBS out of the picture, how do we support the growth of single cells to accelerate workflows and reduce costs? InstiGRO™ CHO is an animal component free cell culture supplement that is specifically designed to help scientists achieve high cloning efficiencies. The product is simply added to the basal culture medium on the day of single cell seeding to support the health of the cells and encourage division into healthy clonal colonies.