Orthogonal Quantification Of CD73 Content And Function Across A Scalable MSC-EV Manufacturing Process

By Stephen Lenzini, PhD, Madeline Cramer, PhD, Elie Zakhem, PhD, and Jon Rowley, PhD

Qualifying CD73 as a critical quality attribute for MSC-derived extracellular vesicles requires analytical methods that connect surface expression to enzymatic function. This poster presents a multi-assay characterization strategy using quantitative capillary western blot, single-vesicle nanoflow cytometry, and a CD73 enzymatic activity assay to track CD73 content and AMP-to-adenosine conversion capacity across each downstream processing step.

You will see how the 0.45 µm filtration step selectively removes larger, CD73-enriched particles and how subsequent steps maintain stability with strong cross-method correlations confirming the relationship between content and function. Access the poster to evaluate whether this framework fits your own process development or regulatory submission strategy.