Application Note

Optimization Of Midstream Cell Lysis And Virus Filtration Steps In An Adenovirus Purification Process

Optimization Of Midstream Cell Lysis And Virus Filtration Steps In An Adenovirus Purification Process

Here we describe screening of detergents for release of adenovirus from HEK293 host cells to replace the traditionally used detergent Triton™. In addition, consumables and conditions that provided the highest virus purity and recovery were selected as a suggestion for a midstream process for adenovirus recovery from a 10 L cell culture, to provide process material ready for the subsequent downstream purification step.

For more information — please visit www.gelifesciences.com/vaccine-platforms

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