The new emphasis on research into viral disease therapies and test kits has placed greater importance on RNA isolation and extraction. Technologies are now available that offer greater simplicity, cost savings, and product availability over commercial kits, while generating equivalent RNA quality and quantity. These approaches employ a single device plus the use of the lab’s own reagents.
The extraction and purification of RNA from various organisms is the primary step for downstream applications such as RT-PCR. Successful use of the extracted and purified nucleic acid requires them to be free of contaminants such as proteins, lipids, or other nucleic acids.
In this paper, we demonstrate that extraction column spin devices can efficiently isolate and purify high-quality RNA and genomic DNA with yields and qualities similar to devices found in commercial kits.