Microcalorimetry For The Biophysical Characterization Of Macromolecules

By Neil Demarse and Morgan Ulrich

Characterizing biological macromolecules is important for understanding their functions and relationships, which empowers the development of new therapies and treatments. Under this branch of macromolecule research, biotherapeutic drug therapy focuses on macromolecular interactions which can lead to disease and/or cell death.

Here, we will learn about microcalorimetry and two techniques for measuring enthalpy and heat capacity changes during reactions. Differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC), are the standard methods for characterizing macromolecules’ intermolecular binding and structural stability.