iPSC-Derived MSCs Demonstrate Comparable Expansion, EV Production, Surface Marker Expression, And Immunomodulatory Potential To Primary MSCs

By Casey Barber, PhD, Lauryl Scott, Mary Doolin, PhD, Terri Willstaedt, and Jon A. Rowley, PhD

If you're scaling MSC manufacturing, the source material you choose shapes everything downstream. This poster presents head-to-head characterization data comparing iPSC-derived MSCs (iMSCs) to primary MSCs across expansion kinetics, surface marker profiles, extracellular vesicle production, IDO-mediated immunosuppression, cytokine secretion, and 3D microcarrier culture. iMSCs sustained lower doubling times across more population doublings, produced higher EV particle concentrations with equivalent CD73 activity, and secreted comparable angiogenic cytokines. The protocol integrates with RoosterNourish and RoosterCollect-EV processing workflows.

Access the poster to evaluate whether iMSCs fit your late-stage manufacturing strategy.