Intensifying Cell Production Processes Through Real-Time Metabolic Control Of Automated ATF Perfusion

Batch and fed-batch processes can limit viable cell density, while periodic sampling may not reveal metabolic shifts soon enough for meaningful intervention. Connecting real-time glucose and lactate measurements with automated ATF perfusion offers a more responsive approach, allowing current metabolic conditions to guide feed and permeate rates.

In a TU Wien NK cell production case study, lactate measurements were used to automatically adjust perfusion parameters throughout expansion. The controlled process achieved more than three times the viable cell density of fed-batch, maintained high viability, and operated within a small working volume.

Compare batch, fed-batch, and controlled perfusion performance side by side, and see how real-time metabolic monitoring can support more adaptive cell culture control.