Impact Of Peptone Supplementation With CHO Cell Lines In Chemically Defined Media

Peptone supplementation has long been a key strategy in enhancing cell culture performance, particularly in CHO cell lines used for biopharmaceutical production. In this presentation, we delve into the benefits of peptone addition to chemically defined (CD) media, highlighting its influence on cell growth, productivity, and protein quality. By analyzing multiple peptone types and blends, we examine how these supplements can address cell culture challenges, such as low productivity and inadequate protein quality. With results from peptone screening and fed-batch assays, we provide insights into optimizing peptone selection to maximize titer yields and modulate glycan structures and charge variants without compromising viability.

This comprehensive analysis serves as a guide to improving biomanufacturing outcomes through strategic peptone supplementation, ensuring better performance and quality in biologics production.