How To Ensure Batch-To-Batch Media Consistency Before Starting Your Bioprocess

RPMI media is one of the most used chemically-defined and protein-free cell media since it works across a variety of mammalian cell lines (e.g., stem cells, T and B cells, hybridomas, HEK293, THP-1 leukemia cells, etc.) grown in suspensions and as monolayer cultures. RPMI media is usually supplemented with fetal bovine serum (FBS) at levels of 1 to 10% to supplement growth factors necessary to sustain specific cell line processes. However, FBS supplemented media presents some issues when there is a desire to perform routine quantitative analysis of the media. The high serum protein contents in the mixed media may interfere with standard chromatography columns for amino acid analysis. Removal of the proteins with precipitation may be required to prepare the samples before analysis with traditional chromatographic platforms. This additional sample prep may affect amino acid recoveries resulting in biased results with conventional analytics approaches for media analysis.

Read how mass spec-based platforms can quickly and confidently screen serum-supplemented media formulations to ensure batch-to-batch media consistency before introducing the cell media to cultures.