Highly Sensitive dPCR Assay For Quantitation Of Lentiviral Proviral DNA In Transduced Cells

The ViralSEQ dPCR Lentivirus Proviral DNA Titer Kit from Applied Biosystems allows for the precise calculation of infectious lentiviral titers and the volume of recombinant lentivirus needed for cell transduction. The kit is also useful in determining vector copy number, a key quality attribute in cell and gene-modified cell therapy development. The assay is compatible with most lentiviral transfer plasmids and targets a conserved region in the lentivirus genome. It provides a wide dynamic range and high sensitivity for the accurate detection of integrated provirus copies. The kit is part of an integrated digital PCR (dPCR) workflow for viral vector characterization during biopharmaceutical manufacturing and is ideal for proviral quantitation in process development and lot release testing.