High Variations Of Analyte Levels Are Detected In Terrific Broth (TB) Media

Terrific broth (TB) is an enriched bacterial media commonly used for high-density cultures of recombinant E. coli for protein expression or plasmid purification. TB media is easily acquired premixed in liquid or dry formulations from commercial sources. Although all sources use the same known formulation to mix the media, the exact concentrations of the individual media components may differ due to the different sources of the raw materials. These differences may affect the culture’s growth or productivity.

Since differences may influence growth or productivities, a quick media screen before the start of culture is advised. Identifying concentration changes across subsequent runs can help build process knowledge to prevent unexpected deviations.