High-Performance Extraction And Quantitation Of Host-Cell Residual DNA

This study presents a high-efficiency method for extracting and quantifying residual DNA from host cells using a semi-automated, high-throughput workflow. The process uses a sample preparation kit based on functionalized magnetic beads and a magnetic particle processing instrument, which can prepare and extract a sample in under two hours. Real-time PCR is used for the quantitative recovery of Chinese hamster ovary (CHO) host-cell DNA, with a wide concentration range of 3–300,000 pg/mL of CHO residual DNA. The extraction efficiency of the PrepSEQ kit and the Thermo Fisher Scientific™ KingFisher™ Flex purification system was found to be high when used with simulated cell culture sample matrices spiked with CHO cell DNA standard. This study demonstrates the effective use of these tools in the extraction and quantification of host-cell residual DNA.