The Thermo Scientific™ Nunc™ Cell Factory™ system is a large multi-layer unit designed for the culture of adherent cells. The design of a Nunc Cell Factory system is similar to a cell culture flask, therefore, a standard cell harvesting protocol may be used. With minor modifications a standard protocol can be optimized for the unique features of the Nunc Cell Factory system.
Incubation, monitoring and the use of a parallel culture:
Establishing a parallel culture in either a Nunc Cell Factory 1 or Thermo Scientifict Nunc flask is recommended as a positive control for cell viability and to assist with determining the proper incubation time for your primary culture. The parallel culture should be maintained under identical conditions, including cell line, cell source, seeding density, medium type, medium volume (mL/cm2), incubation temperature and time."