Genome Engineering Platforms

At Applied StemCell, our expert team offers services using a full suite of advanced and proprietary genome editing platforms to engineer the cell line you need.

Whether your project requires a precise point mutation, a complex knock-in, or stable expression of a large transgene, our team will select and apply the most effective genome engineering technology for your goals. With access to exclusive tools like TARGATT™ technology, as well as trusted systems like CRISPR/Cas9 and MAD7, we can tackle even the most challenging edits in mammalian cell lines.

Learn about the genome engineering platforms we use—each one backed by extensive experience and a track record of success in custom cell line development.

• 1,000+ Gene editing projects completed
• >98% Projects completed to customer specifications
• 8/19 Patents granted/pending

Take a deeper dive into the technologies we use

TARGATT™ technology

Efficient, site-specific knock-in of large DNA fragments into the H11 safe harbor locus. Ideal for stable or inducible expression, even with inserts >50 kb.

CRISPR-Cas9

The go-to gene editing technology for knockouts and point mutations, we also implement the powerful CRISPRa and CRISPRi variants for gene expression control.

Mad7 gene editing

A Cas12a-like enzyme with more straightforward licensing than CRISPR, Mad7 is the tool we turn to for clients engineering cell lines for product development

See what we can do for you with our genome engineering platforms

Cell types*

• iPSCs (healthy or diseased)
• HEK293
• CHO
• Other mammalian cells
• Research grade
• cGMP grade
• Matched research and cGMP grade

Capabilities

• Knock-out
• Knock-in (up to 50 kb)
• Point mutation or single-base editing
• Complex, multi-step cell line engineering
• Multi-gene knock-ins
• Inducible gene expression
• Reliable, consistent expression from the H11 safe harbor site
• Footprint-free edits

Applications

• Disease modeling
• Tissue engineering
• Regenerative & cell-based medicine R&D
• Therapeutic R&D
• Basic research
• Library construction
• Promoter screening libraries
• CRISPR screening libraries
• Cell product cGMP manufacturing