Generating Clonal GFP Cell Lines

Fluorescent reporter constructs are a powerful tool for cell line engineering, allowing easy identification of transfected cells. After transfection, single cells labeled with a reporter are commonly isolated for clonal expansion and generation of recombinant products. Traditionally, generation of clonal engineered cell lines entails a two step process, wherein fluorescent cells are first enriched into a pool using a conventional cell sorter, and then isolated as single clones using manual limiting dilutions. This workflow is not only time consuming, but also has limitations at each step. Namocell’s single cell dispensers are user-friendly, benchtop instruments that operate at low pressure (<2 psi) to preserve the integrity and viability of sorted cells. These systems’ fluorescence detection capabilities allow for easy isolation of transfected cells, yielding clonal cell lines in a single step. Explore the ability of Namocell’s Hana single cell dispenser to isolate and dispense individual GFP-positive cells for clonal expansion.