Fast-Tracking Your MAM Peptide Mapping

You might have seen a lot of publications and webinars discussing ZipChip’s MS compatible charge variant analysis of biotherapeutic mAbs, but did you know you can use ZipChip for peptide mapping too? Some of our earliest customers have been using their systems for peptide mapping and love it because it’s easy, fast, and provides an orthogonal approach to LC for separating tricky analytes like conjugated peptides, complex glycopeptides, isomerized residues, and degradants. You can find applications of peptide maps and peptide analysis with ZipChip in cancer research, anti-viral therapy research, glycoproteomics, and biopharma.

One application of peptide mapping that is getting impossible to ignore is the multi-attribute method (MAM). While still relatively new, MAM is gaining popularity in the biopharmaceutical industry. In its simplest form, MAM is a quantitative peptide map used to directly monitor multiple CQAs of biopharmaceutical drug substances, such as post-translational modifications, sequence variants, and impurities. It is appealing with regards to a quality by design (QbD) approach because it can streamline the characterization workflow and be utilized at many different stages of development from early in discovery to process development and potentially even QC. However, the long LC gradients (sometimes more than 2 hours) often needed to accurately measure vital CQAs presents a potential bottleneck for MAM as it is increasingly used for analysis. ZipChip’s peptide mapping run times are typically about 15 minutes which would result in major time savings for these experiments.