Fab-Oligo Conjugation

Abzena specializes in conjugating oligonucleotides, such as siRNA and ASOs, to antibodies and antibody fragments. This case study highlights our platform process for producing Fab-siRNA conjugates.

Our efficient process uses optimized conjugation chemistry to significantly reduce impurities, which means less time and effort are needed for purification. This is demonstrated by the AEX chromatogram. We also minimize costs by using only a small excess of siRNA.

Even though AEX chromatography can typically be time-consuming and decrease yields, Abzena's process maintains high overall yields (over 70%) and fast processing times. This allows for the complete manufacturing of AOCs in just four days.