Thermo Scientific™ Nunclon™ Delta treated Thermo Scientific™ Nunc™ Cell Factory™ systems is an effective format for the easy and rapid expansion of hMSCs. The Nunclon Delta surface is a fully synthetic chemical surface available with many options of sizes, shapes and surface area. hMSCs were removed from liquid nitrogen and plated directly onto the surfaces to be evaluated. T-25 cell culture flasks were used to determine the optimal seeding density prior to expansion of hMSC on larger formats. The methodology presented here can be applied to the large scale expansion of other cell types for use in various applications using Cell Factory systems.