Evaluating Antibody Fragment Purification Using MabSelect™ VH3 Resin

Capturing antibody fragments and bispecific formats presents unique purification challenges that conventional protein A resins are not designed to address. By shifting affinity from the Fc region to the VH3 domain, a new approach enables selective binding of VH3-class antibody fragments, including human Fab and camelid VHH formats. Purification results highlight strong binding capacities and high recoveries across these fragment types, even under high‑conductivity wash conditions. Explore how buffer composition in intermediate wash steps influences host cell protein clearance, underscoring the role of electrostatic interactions in VH3 binding behavior.

Access the full application note to gain practical guidance for optimizing fragment and bispecific antibody purification, balancing yield and purity while expanding the capabilities of protein A–based workflows.