Enrichment Of High Titer CHO Cells

Cell line generation for monoclonal biotherapeutics has traditionally been limited by the need to screen hundreds of clones from a transfected pool. Recently, fluorescence activated cell sorting (FACS) has improved the throughput of these screens by enabling isolation of high producing clones based on fluorescently-tagged surface markers. One such approach employs a vector enabling “leaky” translation of the IgG heavy chain, resulting in a subset of antibodies being tethered to an IgG transmembrane domain and thus displayed on the cell surface. One limitation of this existing approach is its reliance on a high-pressure cell sorter that often results in low cell viability, and that is not well suited to isolation of subpopulations of rare frequencies (less than 1-3%) due to high dead volume.

Namocell’s single cell dispensers operate at a gentle pressure of less than 2 psi, which preserves viability for clonal outgrowth following isolation. Additionally, Namocell’s patented enrichment sorting mode processes up to 50,000 cells/second, enabling rapid screening of millions of cells and efficient isolation of rare events (less than 1%). Learn how the Namo Single Cell Dispenser was used to isolate high titer CHO cells by dispensing an enriched pool of fluorescently tagged high producers.