Efficient Cell Banking In Seed Train Intensification

By Khalil Essani, Product Line Manager At Single Use Support

According to Khalil Essani, Product Line Manager at Single Use Support, the driving force for seed train intensification in upstream development is a desire to decrease manufacturing timelines and reduce cost of goods, facility space and contamination risk. Seed train processes involve cultivating cells, freezing them and storing them in single-use bags, which can then be thawed and directly inoculated into the bioreactor for each manufacturing run. This reduces the number of seed-train steps and the risk of contamination, while increasing process consistency. Cryopreservation is key to this process, with the most effective method involving the use of high cell density culture in a cryobag. The trend in cell banking is moving towards single-use cryobags, automation, advancements in cryopreservation agents, and technologies for increasing cell densities. In this interview Khalil Essani answers further questions about seed train intensification, cell banking, and the challenges you will confront with cryopreservation processes and improving scalability.