Increasingly, scientists are being challenged to study more biologically-relevant model systems, and this often means imaging thick, demanding 3D samples that may also be alive. To extract the required information, imaging these sample types requires careful selection of the most appropriate imaging technology and flexibility of the experiment’s design within that technique. IRIS and EDGE line-scanning confocal microscopy offer a flexible solution capable of delivering high-quality, rapid imaging of even the most complex 3D live samples.
A choice of technologies
Confocal microscopy is commonly used to refine optical sectioning and improve the signal-to-noise ratio, particularly when imaging thick specimens or samples that scatter light significantly. Two of the most commonly used methods are laser scanning confocal microscopy (LSCM) and spinning disk confocal microscopy (SDCM). However, line scanning confocal microscopy is an alternative method which combines the strengths of an adjustable aperture used in LSCM with the increased speed of SDCM.