Don't Intensify Blindly: Preventing Fouling And Restoring Performance With Targeted Cleaning Of Protein A Resins

Chromatography intensification can improve productivity and resin utilization, but greater efficiency may introduce new risks to process robustness. Protein A capture presents distinct challenges, including balancing loading capacity, residence time, cycle count, column format, and operational consistency. Understanding how these factors interact is essential when determining whether an intensified approach can meet performance expectations without creating unnecessary process complexity or variability.

Explore the practical considerations behind intensified protein A protocols, the tradeoffs that can influence outcomes, and the questions teams should ask before adopting a new strategy.