DoE Methodology For Downstream Purification Of pDNA Of IEX Resins

The rapid expansion of cell and gene therapies and mRNA therapeutics has generated an urgent need for large quantities of highly pure plasmid DNA (pDNA). Unlike conventional protein therapeutics, the production and purification of pDNA present distinct hurdles for downstream processing.

This webinar explores a Design of Experiment (DoE) study focused on optimizing anion exchange (AEX) chromatography for pDNA capture. The study details a systematic approach to evaluating specific POROS AEX resins, determining their dynamic binding capacity (DBC) for a representative pDNA molecule, and pinpointing the optimum process conditions and operating parameters for efficient capture. Discover how this robust, high-throughput methodology can be applied to streamline and accelerate the development of your own pDNA downstream purification schemes. Watch now to learn how to enhance your pDNA purification efficiency.