DNA Purification From PCR Or Agarose Gels Using Pall Nucleic Acid Binding (NAB) Nanosep® Centrifugal Devices

By Craig Tamble and Nadia Kadi

The Pall Nanosep centrifugal device for nucleic acid binding (NAB) incorporates a dual layer silica-based quartz glass fiber media to allow for efficient binding of DNA and RNA, while providing smooth flow and rapid processing of samples. The NAB Nanosep device was originally shown to purify plasmid DNA, genomic DNA, and total RNA from a variety of starting materials. Here we show that the NAB Nanosep device can also be used to purify DNA from polymerase chain reactions (PCR) and from sections of agarose gel using commercially available reagent kits. NAB Nanosep device performance was compared to the silica columns that come with commercial kits for either PCR cleanup or agarose gel extraction.