Datasheet | January 1, 2022

DIBE™ HCP CHO Kit For Validating Host Cell Protein ELISAs

Source: Cytiva
Cytiva1

The Amersham™ HCP DIBE™ CHO kit is designed to measure the antibody coverage of Amersham HCPQuant CHO ELISA on host cell proteins (HCP) from process-derived samples. The kit uses the same polyclonal primary antibodies as the ELISA kit, enabling accurate risk management of HCP impurities in biologics produced in Chinese hamster ovary (CHO) cells from a K1 background. This assay uses fluorescentbased differential in blot electrophoresis (DIBE technology) that simplifies the ELISA assay validation and increases accuracy of HCP coverage analysis. The Amersham HCP DIBE CHO kit was developed by Rockland Immunochemicals, Inc. in collaboration with Cytiva and has been extensively validated. When combined with the Amersham HCPQuant CHO ELISA, Amersham Typhoon™ biomolecular imager, Melanie™ 9 coverage software, and the 2D electrophoresis range, this kit completes Cytiva’s HCP workflow. Together, these products allow for in-house ELISA validation and full control of HCP risk mitigation.

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