Thermo Scientific™ Nunc™ Nunclon™ Delta cell culture products are tested for cell growth and plating efficiency using several different cell lines. Nunc Nunclon Delta products are tested with two cell lines L929, HEL 299 or F2002 and one Primary Chick Embryo cell culture for monolayer formation, plus cell line V79-4 for cloning efficiency. V79-4 is a fibroblast-like cell line derived from the lung tissue of a male Chinese hamster. It has a relatively high plating efficiency and short generation time. This Tech Note describes a procedure for culturing V79-4 cell line on a Nunclon Delta treated surface.