Cryopreserved Autologous Cell Therapy Workflow Processing: Automating Leukopak Processing For Concentration, Washing, And Exchange For Cryostorage

By Robert Bilsky, M.S. , field application scientist

Manual processing of leukopaks introduces variability that remains one of the more persistent quality challenges in autologous cell therapy manufacturing: inconsistent DMSO removal, suboptimal recovery, and donor-to-donor differences that are difficult to control at scale. This poster, presented by Robert Bilsky, M.S., Field Application Scientist at CARR Biosystems, evaluates an automated, closed-process workflow across leukopak washing, concentration, cryopreservation, and post-thaw recovery. Testing across multiple donors and wash media formulations assessed whether automation could deliver reproducible outcomes where manual approaches fall short.

The results address cell population retention, viability maintenance, and washing efficiency in a single integrated workflow. Explore the data to see how the findings bear on scalable, closed manufacturing for autologous therapies.