Comparing Serum-Free To Serum-Supplemented Media With Three Common Vaccine Development Cell Lines

Vero, MDCK, and MRC-5 cells are cornerstone cell lines for producing a wide array of human vaccines, including those for polio, influenza, and measles. While historically cultured in serum-supplemented media, developers are increasingly looking for serum-free alternatives to streamline processes and lower costs.

This application note explores the transition away from serum by directly comparing the growth and productivity of these three essential cell lines in both serum-containing and serum-free media. The study investigates critical performance metrics, such as viable cell density in T-flasks and on microcarriers, as well as viral titers for vesicular stomatitis virus (VSV). Find out how you can achieve comparable or even better results in your vaccine development workflow by reading the full application note.