Article | December 6, 2024

Chromatography Column Packing - MabSelect PrismA™ Resin

Source: Cytiva
GettyImages-658521158 chromatography

Proper column packing is essential in chromatography, ensuring stable, efficient operation and minimizing costly disruptions during bioprocessing. Overly dense beds risk cracking and channeling, while loosely packed beds can compress further, leading to liquid gaps and mixing. These issues reduce efficiency and risk product loss. Properly packed beds maintain stable performance across multiple processing cycles.

MabSelect PrismA™ resin, designed for capturing monoclonal and bispecific antibodies, features high binding capacity, excellent productivity, and alkali tolerance for cleaning with sodium hydroxide. Packing this resin in AxiChrom™ columns ensures optimal bioprocessing performance. AxiChrom™ columns, designed for biopharmaceutical manufacturing, feature mechanical axial compression and Intelligent Packing for precise, reproducible packing across scales. This system calculates parameters like packing factor (PF) and compression factor (CF) to optimize bed height and stability.

Packing protocols vary by column type and slurry properties. Water is the preferred packing solution for MabSelect PrismA™, but other solutions like storage buffers can be used with adjusted packing factors. Intelligent Packing, controlled through software, automates the process for large-scale AxiChrom™ columns, providing precise resin packing and real-time feedback on bed parameters.

Properly packed columns ensure efficient flow, high plate numbers, and long-term bed stability. Verified packing methods yield consistent results, even in large columns, with performance validated through pressure-flow curves and efficiency tests. These approaches enhance reliability and scalability, making AxiChrom™ columns ideal for industrial bioprocessing.

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