Characterizing Process Performance And Robustness In An End-To-End Cryopreserved Autologous CAR-T/TCR Workflow

As autologous CAR-T programs move toward larger scale, manufacturing consistency depends on more than optimizing individual unit operations in isolation. This webinar from CARR Biosystems and BioLife Solutions presents data from a systematic, end-to-end characterization of a cryopreserved leukopak-based CAR-T workflow, covering wash, buffer exchange, cryopreservation, thaw, and final formulation. The evaluation assesses cell health, recovery, viability, and reproducibility while examining how critical process parameters, including flow rate, centrifugal force, and buffer and formulation strategies, influence outcomes and interact across steps.

The result is a practical framework for defining operating ranges and identifying sources of variability in autologous manufacturing. Watch the presentation to learn how this approach addresses one of the field's more persistent process development challenges.