Characterization Of Biopharmaceutical Stability

Protein stability, particularly antibody stability, can be determined by differential scanning calorimetry (DSC) without restrictions on buffers, excipients, or detergents. This characterization method works equally well for novel biotherapeutics, some of which are bispecific antibodies, biosimilars, and modified antibodies including antibody drug conjugates (ADCs). For this example, an ADC was prepared using two different conjugation methods with varying drug loads. Learn how the DSC thermogram was able to detect and quantify the differences in the drug loads with respect to thermal parameters including Tm, Tonset, and enthalpy (ΔH).