Case Study: Developing A 2-Step Chromatographic Process For The Purification Of AAV Vectors

Adeno-associated virus (AAV) has become the workhorse of gene therapy over recent years, leaning extensively on the introduction of purification platforms and capture steps involving immunoaffinity resins such as POROSâ„¢ CaptureSelectâ„¢ AAVX.

POROSâ„¢ CaptureSelectâ„¢ AAVX and POROSâ„¢ 50 HQ were tested for capture and polishing, respectively, of AAV2, AAV9 and a novel engineered capsid to achieve vectors with high purity and significant empty: full ratio improvement.