C-Tag Affinity Tag, From Routine Protein Purification To Use In A cGMP Production Process

By Frank Detmers, Pim Hermans, Remko Clasen and Jessica de Rooij

Epitope tagging is a technique that employs genetic engineering to fuse a known epitope, called an affinity tag, to either the C or N terminus of a recombinant protein. Although the use of an affinity tags implifies the purification and detection of proteins, the tag can alter functionality and stability of the expressed protein and negatively impact final production yields. C-tag is a versatile and user-friendly affinity tag, overcoming the current challenges and limitations of tags in protein purification and detection.