Beyond Standard Protein A Resin

While protein A affinity chromatography is the most commonly used method for monoclonal antibody (MAb) capture in clarified harvest coming from CHO cell cultivation, one significant challenge is the high cost of the media required when using these resins. Therefore, it's crucial to evaluate alternative strategies that may reduce costs and increase productivity without affecting product quality attributes.

In this study, MabCaptureTM C resin has been compared with a Protein A resin used in a DSP platform, showing a higher dynamic binding capacity while keeping the product’s critical quality attributes within acceptable limits. Furthermore, the adsorbent’s robustness and reusability when scaling up makes POROSTM MabCaptureTM the ideal choice for bioprocessing of MAbs.