Beyond High Titers: Expanding Leap-In Transposase® As A Generalized Host Engineering Tool

By Varsha Sitaraman, Renee Saville, Mario Pereira, Sowmya Rajendran, Divya Vavilala, Lynn Webster, Indira Kottayil, Tomasz Sosinowski, Efrain Zarazua, Ferenc Boldog, and Jeremy Minshull

Engineering a mammalian host for both productivity and function often requires more than a single genetic modification. Multiple orthogonal Leap-In Transposase®'s provide distinct integration systems that can be applied sequentially, allowing researchers to add expression cassettes or modify cellular pathways while maintaining the stability of prior integrations.

Experimental examples show the breadth of this strategy, from introducing an antibody payload and reducing fucosylation to knocking down a growth-inhibiting receptor and creating stable lentiviral packaging cell lines. The platform combines controlled transposon integration with genetic circuit design to support durable, multi-step host engineering.

Find out how orthogonal transposases can extend stable cell line development beyond protein titer alone and help address increasingly complex biomanufacturing objectives.