B Cell Isolation For Single Cell Genomics

Single-cell RNA-sequencing (scRNA-seq) allows for dissection of gene expression profiles from individual cells, offering insights into sample heterogeneity and the genomic variability underlying molecular phenotypes. scRNA-seq begins with single cell isolation, which has traditionally been limited to expensive and complicated FACS instruments, often inconveniently housed in core facilities. Namocell’s single cell dispensers offer an alternative to FACS isolation, enabling rapid and reliable deposition of single cells for multiomics approaches. 

Learn how the Namo Single Cell dispenser was used to isolate single activated B cells labeled with both a cell viability dye and a fluorescent, cell type-specific marker. Gene expression was quantified for both resting and activated B cells to identify transcriptional changes associated with the activated cell state.