Application Note

Automated In-Line Buffer Preparation From Readymade Stock Solutions In A mAb Process Step

Source: Cytiva
Automated In-Line Buffer Preparation From Readymade Stock Solutions In A mAb Process Step

Buffer preparation is both time-and space-consuming and can easily become a challenge in biomanufacturing. This application note describes a lean approach to buffer preparation by implementing in-line conditioning (IC). Buffers of different formulations for a mAb chromatography capture step were prepared in an automated, consecutive manner, minimizing manual interactions. To further reduce the time and space required for buffer preparation, ready-made, highly concentrated, low-volume stock solutions were used. Feedback regulation of the final buffers, using  dynamic control, ensured accurate formulations.

For more information — please visit gelifesciences.com/buffer-management

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